User satisfaction is still the first priority in the world of e-commerce and is a very important factor in e-commerce success. Online motorcycle taxis are e-commerce companies that provide online transportation services such as pick-up and delivery. and drop-off, delivery, or grocery shopping in the area. However, in its implementation there are problems that are often experienced by users, including, the application is blank, the application often force closes, loading is slow, paylater cannot be used even though the balance is available, after updating it cannot be used, map points often do not match and navigation is inaccurate so you enter a dead end. So this research was carried out with the aim of measuring the level of user satisfaction with online transportation applications and what variables influence the satisfaction of end users of online transportation applications. The method used in this research uses the End-User Computing Satisfaction (EUCS) method with a sample of 100 online application users in the Banyumas area. The results of this research showed that the level of user satisfaction with online transportation applications was 0.744 or 74.4%, while 25.6% was influenced by other factors not examined in this research. Then, partially and simultaneously, all variables (Content, Accuracy, Format, Ease of Use and Timeliness) have a relationship and have a positive effect on end user satisfaction (user satisfaction) of online transportation applications