Aging is a
progress
ive and multifactorial process,
physiological changes
occur in various tissues in disorders of biological function and repair
mechanisms. The aging process in the skin involves the epidermis, dermis and
subcutaneous tissue. The influential factors
for accelerating the occurrence of
premature aging are UV rays from sunlight causing changes in the connective
tissue through the formation of lipid peroxide, changes in cell contents and
activation of enzymes that cause aging such as collagenase and elast
ase enzymes,
and the presence of reactive oxygen species (ROS). This study was to examine the
anti
-
aging activity of secondary metabolites contained in the perfume (Azolla
microphylla K.) with the determination of total phenolic content, total flavonoids
a
nd anti aging test using the method of Collagenase Inhibitor. Determination of
total phenolic levels using the Folin
-
Ciocalteau method with a comparison of
gallic acid and quercetin comparators for the determination of total flavonoid
levels. Collagenase e
nzyme inhibitors were carried out
to determine the ability of
herb extract to prevent premature aging by inhibiting the collagenase enzyme in
the skin. The results of the determination of total phenolic content and total
flavonoids f
or pumice herb extracts
were 17.
28 ± 0.27% b / b f
or total phenolic
and 0.9
97 ± 0.49% b / b for total flavonoids. The anti aging test results on the
collagenase enzymes of herbaceous herb extract showed 65.50% inhibition
compared to Phenanthroline Inhibitors.