Background : sirsak leaf can activity as antioxidant so it can be used as immunostimulator. Antioxidant it self plays an important role to increase the functions of immune cells. The compounds which become the immunostimulator are flavanoid and tanin.
Objective : This research was to determine the activity of the ethanol extract of sirsak leave (Annona muricata L) as immunostimulator and to identify the secondary metabolic compound in the ethanol extract of sirsak leaf (Annona muricata L).
Methods : This research is pure experimental one with randomized block design. The chemical substances used were methanol, ethanol dissolvent, stimuno, levamisol HCl, Giemsa, CMC Na and chloroform. The experimented animal was a Swiss strain male mouse aged 2-3 months and 20-30 g body weight. Group I was treated by giving Levamisol HCl 2.5 mg/kg of body weight. Group II was given Stimuno 9.1 mg/kg of body weight. Group III was given Na CMC 1%. Group IV was treated by giving the ethanol extract of sirsak leaf 35 mg/kg of body weight. Group V was given the ethanol extract of sirsak leaf 70 mg/kg of body weight. The last group, Group VI, was treated by the ethanol extract of sirsak leaf 140 mg/kg of body weight.
Result : This research employed a Swiss strain male mouse aged 2-3 months and 20-30 g body weight as the experimented animal. The observed parameters were the number of macrophages which phagocyte latex and the amount of latex which was phagocyted by macrophage. In order to know whether there was significant difference or nor from the positive control, ANOVA test was employed with level of significance 95%. The result of the number of macrophage which could phagocyte latex was 11.736 > 3.11 (F table > F counted) and the value of P = 0.000. While, the amount of latex which was phagocyted by macrophage was F counted (10.472) which was bigger than F table (3.11) and the value of P = 0.000. This shows that there was a significant difference between the positive control and negative control and the treatment. Tukey test also shows that there was a real difference between the dosage of 35 mg/kg of body weight with the positive control (levamisol 2.5 mg/kg of body weight and stimuno 9.1 mg/kg of body weight), and for the dosage of 70 mg/kg of body weight and 140 mg/kg of body weight, there was no real difference or similar to the positive control. The compound identification shows that sirsak leaf contain flavanoid and tanin.
Conclusion : The ethanol extract of sirsak has imunostimulator activity if the dosage is 70 mg/kg of body weight and 140 mg/kg of body weight. The identification result of sirsak leaf with thin layer chromatography shows that the sirsak leaf contain flavanoid and tannin.