MUTDYARINI, AYUDITA NUR (2020) PENETAPAN PARAMETER MUTU SIMPLISIA AKAR PURWOCENG (Pimpinella pruatjan Molk) DAN AKTIVITAS PENANGKAP RADIKAL BEBAS. Bachelor thesis, UNIVERSITAS MUHAMMADIYAH PURWOKERTO.
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Abstract
Background: The use of traditional medicines needs to be utilized and developed in standardization. Purwoceng plant (Pimpinella pruatjan Molk) is a native plant of Indonesia which has many uses for health which is endemic to grow in mountainous areas. Purwoceng root contains secondary metabolites such as coumarin compounds, saponins, sterols, alkaloids, some sugar compounds, and stigmasterol compounds. his study, quality parameters were determined for Purwoceng root simplicia.in addition, testing free radical scavenging activity from Purwoceng root ethanol extract. Method: The determination of quality parameters of purwoceng root simplicia is carried out in accordance with Indonesian Herbal Pharmacopoeia guidelines. Free radical capture activity test using DPPH method with purwoceng 0.01% w / v root extract concentration and positive control of BHT. In addition, test identification of groups of compounds using Thin Layer Chromatography (TLC) method. Result: Shows that Purwoceng root extract has organoleptic characteristics in the form of whitish brown powder with a bitter taste and characteristic odor. Ethanol soluble extract content of 2.08% ± 0,21, water soluble extract content of 6.68% ± 0,31, drying shrinkage of 7.86% ± 4,84, and water content of 5.18%. TLC tests obtained compounds detected were Alkaloids, Saponins, and Flavonoids. The free radical capture test of purwoceng root extract showed an IC50 value of 131.3015 μg / mL and a positive control of ICHT BHT of 25.224 μg / mL. The free radical capture test of purwoceng root extract showed an IC50 value of 133.56 μg/mL and a positive control of ICHT BHT of 25.224 μg/mL. Conclusion: Simplicia Purwoceng roots have good parameters. The ethanol extract of Purwoceng root had free radical scavening activity with IC50 of 133,56 μg/mL lower than the positive control of BHT.
| Dosen Pembimbing: | WAHYUNINGRUM, RETNO | unspecified |
|---|---|
| Item Type: | Thesis (Bachelor) |
| Uncontrolled Keywords: | Purwoceng,roots, quality, free radical, DPPH |
| Subjects: | R Medicine > RS Pharmacy and materia medica |
| Divisions: | Fakultas Farmasi > Farmasi S1 |
| Depositing User: | wulan |
| Date Deposited: | 05 Aug 2022 04:01 |
| Last Modified: | 14 Nov 2024 01:29 |
| URI: | http://repository.ump.ac.id/id/eprint/13326 |
