Carica plants have many benefits in daily life. Unfortunately, the plants are still planted conventionally which takes more time for cultivation. Therefore, this research aimed to obtain the in vitro endosperm callus induction using the combination of BAP and 2.4-D. The design of this research was randomized block design with 2 factors and 3 repetitions, while the concentrations consisted ofBAP 0mg/l, 2mg/l,4mg/l and2.4-D 0mg/l, 1mg/l, 2mg/l, 3mg/l, 4mg/l, 5mg/l. The variables observed included the callus induction days, the callus growth percentage, the explant browning percentage, the callus textures, and colors. The results showed that the concentration of BAP 0 mg/l was the most effective one in inducing the callus (25, 32 days), while on the 2.4-D treatment, 2.4-D 3 mg/l (20,50days)was the most effective concentration. The combination of BAP 0 mg/l + 2.4-D 1 mg/l concentration showed the fastest growth of callus (17, 50 days), the concentration of BAP 0 mg/l showed the highest percentage of the growth (0.06 %), and the 2.4-D concentration treatment showed the most callus growth that was 2.4-D 1 mg/l and2.4-D 2 mg/l (0.09 %). Meanwhile, the combination of BAP 0 mg/l + 2.4-D 1 mg/l concentration showed the highest callus growth(5.00%), the treatment with BAP 2 mg/showed the highest percentage of explant browning (4.07%), while the treatment of 2.4-D concentration showed that the most explant browning was on the concentration of2.4-D 1 mg/l (4.43%). Lastly, the combination of BAP 0 mg/l + 2.4-D 1 mg/l concentration produces the highest percentage of browning explant(5.00%). The callus texture produced was crumb and the colors produced were white to brown.