Breast cancer is one of the most commonly diagnosed cancers in women. 5 Flouroulacil (5-FU) is used as a chemotherapy agent for cancers include breast cancer but it’s less effective and can cause high side effects if the dose is increased. Herbal supplements are now widely used to maintain health even for medical treatment and are considered have no side effects. Herbal Drops Medicine (HDM) is a popular herbal supplement on the market consisting of natural compounds that have anticancer effects. This study aims to determine the active combination contained in herbal drops with TLC-Densitometry, and to knows anticancer activities combined 5-FU with the MTT test (3- [4,5-dimethylthiazole-2yL] -2,5-diphenyltetrazolium bromide) method in T47D breast cancer cells and proliferation test with doubling time. The TLC profile of HDM has similarities to curcumin and quercetin TLC standard profiles with Rf values of 0.73 (curcuminoid), 0.65 (desmethoxykurcumin) and Rf 0.70 (quercetin). HDM shows a cytotoxic effect with IC50 440, 792 μg / mL while 5-FU has a cytotoxic effect with IC50 259,841 μg / mL. The combination of JMT 55 μg / mL (1/8 IC50) with 5-FU 32 μg / mL (1/8 IC50) have a strong synergistic effect with a CI value of 0.113. The JMT and 5-FU treatments have proliferative inhibitory activity starting after 0 to 72 hours when compared to a single treatment. JMT has anticancer activity and has a potential as a 5-FU chemotherapy agent in T47D cancer cells.