This research aimed to know the effect of self-efficacy, entrepreneurial education and income expectations against the interest in entrepreneurship. Research conducted at the Faculty of Economics and business of Muhammadiyah University of Purwokerto with the entire population of students of the Faculty of Economics and business of Muhammadiyah University of Purwokerto who have attended courses in entrepreneurship. The sample data is retrieved based on the number of students who are still active in College from the year 2014-2016 (Student semester 3, 5 and 7). Sampling using stratified random sampling technique proportioned and uses the Slovin's formula with tinggat trust 90% and acquired 94 respondents. The data collected by questionnaire and data processed using the Statistical Program for Social Science (SPSS) 22.0 For Windows. Analysis tool used is Test validity, Reliability Test, test the assumptions of classical and Linear Multiple regression analysis. The research results showed that entrepreneurship education has significantly positive effec towerd entrepreneurship interest then the income expectations has significantly positife effect toward entrepreneurship interest.