RISSA EKA SUSANTI. Imunostimulant Test of Rosela Flower Ethanolic Extract
(Hibiscus sabdariffa L.) to the Macrophaga phagocyitosis Activity and Flavonoid
Compound Detection.
Under direction of DINIATIK and SUPARMAN.
Background : Human need imunostimulant for increase the quality of imune system to
confront some infection deseases. Imunostimulant is the materials which can increase
performance of imune system element of human. Flavonoid in plants have function as
imunostimulan. Rosela flowers had contains flavonoid so that its expected to have
imunostimulant.
Objective : The aims of this research was to prove that Rosela flower ethanolic Extract
(Hibiscus sabdariffa L.) have imunostimulant effect represented by macrophaga
phagocytosis activity, and determine the seconda rymetabolic compounds contained in
ethanolic extract of Rosela flower.
Methods : The researh used Staphylococcus aureus as respons imune model. Its function
as in vivo antigen of macrophaga phagocytosis imunostimulator and latex as in vitro
antigen. The phagocytosis ablity was shown by activities and capacyties of macrophag in
latex phagocyt. The plant compounds analized used Tin Layer Cromatografy.
Result : The observe result of macrophaga morphology after infect by S. Aureus in
Levamisol®, Stimuno® and extract dose 100 mg/KgBB, 200 mg/KgBB and 400 mg/KgBB
showed presence of macrophaga activated. The increasing of extract dose caused the
increasing of macrophaga number which phagocyt of latex and the number of latex which
was phagocyt by macrophaga. The macrophaga phagocytosis ablity of flower ethanolic
extract in dose 400 mg/KgBb weren’t deference with Stimuno® 9,1 mg/KgBb. Tin Layer
Cromatografy showed that flavonoid compound contained in Rosela flower Petals
ethanolic extract.
Conclution : Rosela flower Petals ethanolic Extract (Hibiscus sabdariffa L.) had
imunostimulant effect to the macrophaga’s phagocytosis. Rosela flower Petals ethanolic
Extract (Hibiscus sabdariffa L) contain flavonoid.
Key words : Imunostimulant, phagosytosisy, Rosela flower