The tratment of 5-Fluorouracil (5-FU) in body is metabolized more than
80% of the dose inactivated by the liver, almost 15-20% is eliminated in the urine
and only a small portion remains available to provide anticancer action. 5-FU is
known causes cardiotoxic, causing serious side effects of cardiomyocytes which
followed by heart failure. Other side effects that arise are diarrhea, dehydration,
abdominal pain, nausea, neutropenia, stomatitis, and hand-food syndrome. This
study was attempt a research for co-chemotherapy agents that can increase the
efficacy and minimize the side effects of 5-Fluorouracil. By determining the
compound content, a test of single cytotoxic and cytotoxic combination effect of
ethyl acetate fraction Eleutherine bulbosa on T47D cells. Phytochemical
screening was carried out by thin layer chromatography. While the cytotoxic test
was performed on T47D cells using the MTT assay method with an incubation
time of 24 hours. Phytochemical screening results The dayak onion ethyl acetate
fraction contains alkaloid, phenolic, tannin, steroid, saponin, and terpenoid
compounds. The ethyl acetate fraction Eleutherine bulbosa has a moderate
cytotoxic effect on T47D cells with IC50 value is 102,43 μg/mL. The combination
of ethyl acetate fraction Eleutherine bulbosa with 5-FU at high and low
concentrations caused an antagonistic effect on T47D cells (CI 1,54 - 2,09).