The purpose of this study is to examine the effect of service quality and customer trust on customer loyalty with customer satisfaction as an intervening variable. This research uses non probability sampling method, with purposive sampling technique is a method of determining the sample based on certain criteria. The population in this study were customers of DestSkin Beauty Clinic Purwokerto. This research sample was 80 respondents. The research method used in this study is validity test, reliability test, classic assumption test, multiple regression analysis, coefficient of determination, F test, t test and sobel test. Service quality and customer trust have a significant positive effect on customer satisfaction both simultaneously and partially, while service quality and customer trust have a significant positive effect on customer loyalty both simultaneously and partially. The results of the sobel test show that customer satisfaction can mediate service quality towards customer loyalty, customer satisfaction can mediate customer trust in loyalty.