This study aims to determine and analyze the effect of job characteristics, competence and teamwork on employee performance at Perumda Air Drinking Tirta Satria Purwokerto. Sampling using purposive sampling method through a questionnaire. The sample used amounted to 160 employees. The analysis technique used in this study uses the Statistical Package for the Social Sciences (SPSS). The analysis used to analyze is the Determination coefficient test (Adjusted R2), Multiple Linear Regression Analysis, F test and t test. The results of this study show that: 1) Job characteristics, competence and teamwork together have a positive and significant effect on performance of employees at Perumda Water Drinking Tirta Satria Purwokerto. 2) Job characteristics and competencies have a positive and significant effect on employee performance at Perumda Air Drinking Tirta Satria Purwokerto. 3) Teamwork has no effect and is not significant on the performance of employees at Perumda Water Drinking Tirta Satria Purwokerto. The results of the determination test (R2) show that the influence of Job Characteristics, Competence and Teamwork on Employee Performance is 48.6%