This study aimed to determine the diversity of arbuscular mycorrhizal fungi in pineapple plant rhizosphere (Ananas comocus L. merr) in three villages in Belik sub-district, Pemalang. This research was carried out in 3 places, namely pineapple land in Belik Sub-District Pemalang Regency, Integrated Laboratory of Universitas Muhammadiyah Purwokerto and Basic Agrotechnology Laboratory of Faculty of Agriculture, Universitas Muhammadiyah Purwokerto. The study lasted for about 5 months starting from January 2019 to May 2019. This study used a survey method with sampling which was done intentionally or purposive sampling based on homogeneity. The data obtained were analyzed descriptively qualitatively to identify Arbuscular Mycorrhizal Fungi. The observed variables were spore diameter, spore color, number of spore walls, presence or absence of typical structure and spore shape, spore population, and intensity of root infection. The results showed that there were 4 genera of Arbuskula Mycorrhizal Fungi in Belik, Beluk and Bulakan Villages, Belik Sub-District, Pemalang, namely Glomus, Gigaspora, Acaulospora, and Scutellospora.