Aging is the process of slowly disappearing the ability of tissues to repair or
replace themselves and maintain their normal structure and function. The most
common cause of premature skin aging is due to exposure free radicals in the
form of ultraviolet (UV) rays called photoaging. Exposure to UV light causes
changes in connective tissue through the formation of lipid peroxide, changes in
cell content and activation of enzymes that cause aging such as the enzymes of
collagenase and elastase, and the presence of Reactive Oxygen Species (ROS).
The aim of this study was to determine the anti aging activity of purified extracts
of Kepel (Stelechocarpus burahol (BI.) Hook F. & Th) extracts by inhibiting the
collagenase enzyme. Ethanol extract (EESL) and Kepel leaf purified extract
(PESL) determined antioxidant activity, SPF value, total phenolic and flavonoid
levels and collagenase enzyme inhibition test. The result of determining
antioxidant activity with DPPH method obtained IC50 value of vitamin C
4.59±0.046 μg/ml, EESL 9.53±0.062 μg/ml and PESL 5.95±0.048 μg/ml. FRAP
method obtained IC50 values of vitamin C 6.66±0.150 μg/ml, EESL 16.13±0.156
μg/ml and PESL 24.53±0.114 μg/ml. BCB method obtained IC50 values of
quersetin 61.87±8.808 μg/ml, EESL 134.25±4.478 μg/ml and PESL 116.82±9.982
μg/ml. The SPF value of PESL at a concentration of 200 ppm was 5.70. Total
phenolic content of EESL was 56.89 ± 0.0006 μgGAE/g and PESL was 61.14 ±
0.0001 μgGAE/g, while the total flavonoid content of EESL 30.07 ± 0.0001
μgQE/g and PESL were 40.47± 0.0001 μgGAE/g. Anti aging activity of PESL
showed an inhibition of 73.59% compared to Phenanthroline.