Cutchery lily (Kaempferia galanga L.) is one of the potential herbs to be used as medicine because its rhizome contains saponins, flavonoid, phenol, and essential oil. In vitro culture is not only used to propagate plants but
also to produce callus. A callus is a cell mass which is not differentiated yet and it consists of irregular cells. The formation and growth of callus can be triggered by giving the combination of auxin and cytokinin plant growth
regulators.
The research on the effect of giving plant growth regulators of 2.4 D (2.4-dichlorophenoxyacetic) and BAP (6-benzyl amino purine) on the callus induction of rhizome and shoot explants of cutchery lily (Kaempferia galangal L.) had been conducted in the Basic Agrotechnology Laboratory and Plant Engineering Laboratory, Universitas Muhammadiyah Purwokerto during December 2018 – April 2019. This research applied Completely Randomized Design for each rhizome and shoot explant. This research used 2 factors, namely giving some concentration of 2.4 D and BAP. Each factor consisted of 3 levels, so it was gained 9 treatment combinations with 3 repetitions in each treatment. The observation variables covered the percentages of contamination and browning, contaminant source, days of callus' emergence, callus morphology, callus' color,
and fresh callus' mass.
The results of the research showed that the combination of plant growth regulators of 2.4 D and BAP significantly affected the variables of days of callus' emergence and fresh callus' mass on the shoot explant. The combination of plant growth regulators of 2.4 D 1 ppm + BAP 0.3 ppm concentration had the most optimum effect because it had the fastest average of days of callus' emergence, which was 28 hst, while the average of fresh callus' mass in this combination was 0.29 gram. The callus formed here was structurally soft and white to brownish-white in color.