AKTIVITAS ANTIVIRUS EKSTRAK ETANOL HERBA PATIKAN CINA (EUPHORBIA THYMIFOLIA L. ) TERHADAP VIRUS NEWCASTLE DISEASE DANPROFIL KROMATOGRAFI LAPIS TIPISNYA

ANDESTI, PRIZKY (2013) AKTIVITAS ANTIVIRUS EKSTRAK ETANOL HERBA PATIKAN CINA (EUPHORBIA THYMIFOLIA L. ) TERHADAP VIRUS NEWCASTLE DISEASE DANPROFIL KROMATOGRAFI LAPIS TIPISNYA. Bachelor thesis, UNIVERSITAS MUHAMMADIYAH PURWOKERTO.

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Abstract

Test has been done about antiviral activity ethanolic extract of Patikan cina herba (Euphorbia thymifolia (L.) ) against Newcastle Disease Virus and Profile of Thin Layer Chromatography. The aim of this research was exploring the potency of ethanol extract of Patikan cina herba as antiviral against Newecstle Disease Virus, to know the content of the extract that has the potential antiviral activity, and Profile of Thin Layer Chromatography. Antiviral activity were observe by hemagglutination test. This study used the embryo chicken eggs at age of 9-11 days were classified at the test subjects for the concentration 50 μg/ml, 100 μg/ml and 150 μg/ml. Each groups were given suspension virus then inoculated to embryonated chicken eggs and incubated at 37 0C for 2-3 days. The observe data is the percentage of antiviral resistance. The data is analyzed statistically with one-way ANAVA with 95% confidence level. The identification ethanolic extract of Patikan cina herba compounds used thin-layer chromatography (TLC). The result antiviral activity of Patikan cina herba ethanolic extract against Newcastle Disease Virus at concretation of 50 μg/ml, 100 μg/ml,150 μg/ml and positive control showed average concretation of 58,3%, 75 %, 66,7 % and positive control 75%. The result profil of thin layer chromatography showed that ethanolic extract of Patikan cina herba (Euphorbia thymifolia (L.) ) contained flavonoid and saponin compound. Ethanolic exstract of Patikan cina herba (Euphorbia thymifolia (L.) at the concentration of 50 μg/ml has the precentage of antivira lresistence on the growth of virus 58,3%, conntrol of ethanolic solvent doesn’t has antiviral resistance because the result is equal with the virus control. At the 100 μg/ml as the precentage of antiviral resistance on the growth of virus 75%, at the concentration of 150 μg/ml has the precentage of antiviral resistance on the growth of virus at 66,7%. Ethanolic extract of Patikan cina herba (Euphorbia thymifolia (L.) has the antivira resistance on the growth of virus (66,7%, 75%) then a smaller which has osaltamivire the positive control (89,58%).

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