Sambirata Village, Cilongok Sub-District, Banyumas Regency, has the Potential for Natural Resources, such as agricultural, tourism, and water source. The study aimed to determine the distribution, connectivity, and potential of natural resources in Sambirata Village, Cilongok District, Banyumas Regency, and to identify the potential of natural resources in Sambirata Village, Cilongok District, Banyumas Regency. This study implemented the observation method. The field observation method was used to obtain primary data: observing coordinate points and evaluating the assessment sheet in Potential Natural Resources. The sample was obtained from purposive area sampling, which aimed to obtain a representative sample under the criteria. Data collection techniques were GPS, Geographic System software, cameras assisted materials from several maps, and satellite imagery taken from SAS planets. Data processing included creating maps of land use, distribution, and connectivity. The findings indicated that the distribution of natural resources was in the southern part of Sambirata Village, and the connectivity of tourism potentials was medium. In contrast, the connectivity of agricultural potential and potential of water resources was high, tourism potential was in medium, and agricultural potential was in the medium. Water potential resources were inappropriate for direct consumption