This study aims to examine and analyze the effect of job training and work experience on the work ability and performance of employees of Java Heritage Hotel Purwokerto. The population used in this study were all employees of Java Heritage Hotel Purwokerto. The sample of this research is 101 employees with the sampling technique using simple random sampling technique. For statistical tests and data processing is done using Partial Least Square (SmartPLS). The results of this study indicate that: 1) job training has a significant positive effect on work ability, 2) work experience has a significant positive effect on work ability, 3) job training has a significant positive effect on employee performance, 4) work experience has a significant positive effect on employee performance. , 5) work ability has a significant positive effect on employee performance, 6) work ability is able to mediate the effect of job training on employee performance, 7) work ability is able to mediate the effect of work experience on employee performance. The results of the coefficient of determination (R2) show that the effect of job training, work experience and work ability on employee performance is 68,8%.