Temu hitam (Curcuma aeruginosa Roxb.) contains saponins, flavonoids,
polyphenols and essential oils and already known has antioxidant properties.
Previous study show high potency antioxidant of aethanolic extract of temu hitam.
This study aims to determine the antioxidant acticity of ethanolic extract and
purified extract of temu hitam using DPPH and FRAP methods. Use prepare
purified extract using two different solvent, ethyl acetate-hot water and n-hexant.
We have 2 (two) purified extract (PE), PE1 and PE2. PE2 has total phenolic
content higher (69.317 ± 1.652 g GAE/g samples ) than PE1 and ethanolic
extract (64,518 ± 0,578 g GAE/g samples and 48,106 ± 0,378 g GAE/g
samples, respectively). All ethanolic extract show study % very strong antioxidant
protected based on DPPH and FRAP method. Result of antioxidant
The results showed that the second purified extract had the largest total phenolic
content of 69.317 ± 1.652 g GAE/g samples, and the smallest phenol content in
ethanol extract 48.106 ± 0.378 g GAE/g samples. The antioxidant activity in
DPPH method obtained the lowest IC50 value pf 16,229 g/ml in the first purified
extract., which means that the antioxidant activity was very compare to IC50 7,437
g/ml of ascorbic acid as a comparing agent and the antioxidant activity of the
FRAP obtained IC50 value 60,336 g/ml in the second purified extract which
means it has strong antioxidant activity but the IC50 value is weaker than the
antioxidant activity of ascorbic acid as a comparing agent is 10,578 g/ml. The
result of this study can be concluded that the ethanol extract and purified extract
of temu hitam had a significant differences. Total phenolic levels of purified
extracts are higher than before it is not purified yet. The first purified extract temu
hwas effective as an antioxidant with the DPPH method while the second purified
extract was effective as an antioxidant with the FRAP method.