This study aims to examine the effect of entrepreneurship education, self-efficacy, income expectations, and academic support on interest in entrepreneurship. This research was conducted at the Faculty of Economics and Business, Muhammadiyah University of Purwokerto with a population of all students of the Faculty of Economics and Business, Muhammadiyah University of Purwokerto who have taken entrepreneurship courses 4. The sample data was obtained based on the number of students who are still actively studying from 2016-2018 (4th semester students, 6 and 8) with a total of 1,307. Sampling using random sampling techniques and using the Solvin formula so that the data obtained by 110 respondents. The analytical tool used to test the hypothesis is Multiple Linear Regression analysis. The results of this study indicate that entrepreneurship education, self-efficacy, and income expectations have a significant positive effect on interest in entrepreneurship. Meanwhile, academic support has no effect on interest in entrepreneurship.