PENGARUH PEMBERIAN EKSTRAK BAWANG PUTIH (Allium sativum) TERHADAP PENINGKATAN IMUN NON SPESIFIK LELE DUMBO (Clarias gariepinus)

MAHARANI, TIARA SUKMA (2021) PENGARUH PEMBERIAN EKSTRAK BAWANG PUTIH (Allium sativum) TERHADAP PENINGKATAN IMUN NON SPESIFIK LELE DUMBO (Clarias gariepinus). Bachelor thesis, UNIVERSITAS MUHAMMADIYAH PURWOKERTO.

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Abstract

In African catfish cultivation, there are often obstacles to disease, including MAS (Motile Aeromonas Septicemia), which can cause crop failure. Generally, fish farmers cope it with antibiotics. However, improper use of antibiotics can lead to resistance, residual deposits in the body, and environmental pollution. A better alternative treatment is by natural immunostimulants such as garlic extract. This study aimed to determine the effect of garlic extract given through feed on the non-specific immunity of African catfish. The experimental design used in this experiment was a completely randomized design (RAL). The treatments tested were garlic extract at a dose of 0 mg/kg of feed as a control (P0), 10,000 mg/kg of feed (P1), 20,000 mg/kg of feed (P2), 30,000 mg/kg of feed (P3). The main parameters observed were the percentage of hematocrit, leucocrit, and differential leukocytes (lymphocytes and monocytes), while the supporting parameters observed included pH, temperature, and oxygen solubility. Data were analyzed using the F-test with a significant level of 95%, followed by the Duncan Multiple Range Test (DMRT) at the 95% significant level. The results discovered that garlic extract administration through feed contributed a significant effect on improving the percentage of hematocrit, leucocrit, and leukocyte differentials, both lymphocytes, and monocyte. The treatment of optimum garlic extract in increasing non-specific immunity of African catfish (Clarias gariepinus) is P2 treatment with a dosage of 20,000 mg/kg of garlic.

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