The combination of dexchlorpheniramine maleate and dexamethasone tablet is an antihistamines and corticosteroids combination. A tablet with more than one active substance will be more difficult to assay because it requires separation beforehand. This research sought to determine whether the zero crossing UV-Vis spectrophotometry method can be used to determine the levels of dexchlorpheniramine maleate in a mixed preparation tablet containing dexchlorpheniramine maleate and dexamethasone. The method can determine the content of active substances in mixed preparation tablet without separating. Method: This research was conducted using zero crossing UV-Vis spectrophotometry method. The validations of the analytical method were performed through linearity, LOD & LOQ, precision, and accuracy tests. The solvent used was methanol, with the analytical wavelength of dexchlorpheniramine maleate used in 212 nm. Result: The results revealed that the proposed method satisfied the requirements and had good linearity of 0.9976 (r > 0.99), LOD & LOQ of 0.62 μg/mL and 1.879 μg/mL. The analytical method provided a precision score meeting the requirement (RSD < 2%) wherein the intra-day precision reached 0.784% and the inter-day precision was 1.479% and obtained 80-120% recovery range for accuracy. The result of determining the levels of dexchlorpheniramine maleate showed that the percentage of levels satisfied the requirement of 90-110%. Conclusion: It can be concluded that this method can be used to determine the levels of dexchlorpheniramine maleate in mixed preparation tablet of dexchlorpheniramine maleate and dexamethasone.