PENGARUH PEMBERIAN AUKSIN DAN SITOKININ PADA PROLIFERASI KALUS KENCUR SECARA IN VITRO

WILLYANVAN, - (2018) PENGARUH PEMBERIAN AUKSIN DAN SITOKININ PADA PROLIFERASI KALUS KENCUR SECARA IN VITRO. Bachelor thesis, UNIVERSITAS MUHAMMADIYAH PURWOKERTO.

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Abstract

Kaempferia galangal (kencur) is one of the many rhizome plants grown in Indonesia both in the lowlands and mountain areas. It is also known as a medicinal plant which can cure various diseases because of its usefulness. Kencur is generally cultivated by conventional way which still does not meet the demand of kencur market nationally, so the propagation through callus culture method by in vitro may be an alternative to provide kencur seeds in large quantities and uniformities. From the research on the effect of auxin and cytokines concentration on the proliferation of Kencur callus, the callus selected was the one which was ready to regenerate and culture in MS medium combined with NAA (naphthaline acetate acid) and Kinetin (furfuril aminopurin) with concentration of NAA 0, 0.5, 1, 1.5 mg /l and Kinetin 0, 1, 2 mg/l. Observation variables included were wet weight, callus color, callus texture, percentage contamination and percentage of success. The results of NAA and Kinetin significantly affected the wet weight of callus, with the best combination average on the treatment of A1B1 or 5.45 grams. Callus colors obtained were brown, green, green white, white, and white brown. Texture created i.e. compact and crumb. Contamination level was between 0-33%. The research results concluded that Auxsin and cytokines both single and combination affected on the color formation, texture, success of callus planting, contamination of callus and callus weight.

Item Type: Thesis (Bachelor)
Additional Information: Pembimbing: Anis Shofiyani., SP., MP., dan Hamami Alfasani Dewanto., SSi., M.Si.
Uncontrolled Keywords: Tissue culture, NAA, Kinetin, Kaempferia galangal
Subjects: S Agriculture > S Agriculture (General)
Divisions: Fakultas Pertanian > Agroteknologi
Depositing User: Iin Hayuningtyas
Date Deposited: 15 Nov 2018 03:39
Last Modified: 15 Nov 2018 03:39
URI: http://repository.ump.ac.id/id/eprint/8081

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