UJI EFEKTIVITAS MINYAK ATSIRI KEMANGI (Ocimum x africanum Lour.) TERHADAP PERTUMBUHAN Aeromonas hydrophila GPl-04 SECARA IN-VITRO

RATNASARI, AMALIA (2014) UJI EFEKTIVITAS MINYAK ATSIRI KEMANGI (Ocimum x africanum Lour.) TERHADAP PERTUMBUHAN Aeromonas hydrophila GPl-04 SECARA IN-VITRO. Bachelor thesis, UNIVERSITAS MUHAMMADIYAH PURWOKERTO.

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Abstract

Fish breeding, especially bream was growing very fast. It was supported by the people requests were growing each year. However, there were some problems to the breeding such as Motil Aeromonas Septicemia (MAS) caused by Aeromonas hydrophila bacteria. One of the ways to cure the bacteria was antibiotic. It was effective, meanwhile it caused resistance to bacteria and there was residue to the fish. Therefore, it was needed to have natural substance to cure MAS such as ocimum. The research was aimed to observe the effectiveness of ocimum aetheric oil to the growth of Aeromonas hydrophila GPl-04. The research was done in microbiology lab University of Muhammadiyah Purwokerto and taxonomy la University of Jenderal Soedirman in four months. It was experiment research through Complete Random Design (RAL) with 7 types of treatment, which was 20%, 40%, 60%, 80%, 100%, add by 2 controls, negative (0%) and positive (Chloramphenicol). Each of the treatment was repeated three times. Ocimum aetheric oil was obtained from steam distillation. The parameter observed was blocked zone producted by ocimum aetheric oil and chloramphenicol. To analyze the data, it was done through Analysis of Variance (ANOVA) with 95% level of significance and continued with lowest differentiate test (BNT) with 95% level of significance. The research showed that ocimum aetheric oil could hinder the growh of A. hydrophila GPl-04, and there was difference among the treatmens. However, based on CLSI, it was classified as resistance category and not effective if it was compared to chloramphenicol antibiotic. The comparative between ocimum aetheric oil and chloramphenicol antibiotic was 52,576 : 1 (ppm/unit).

Item Type: Thesis (Bachelor)
Additional Information: Pembimbing: Dini Siswani Mulia, S.Pi., M.Si. dan Drs. Arief Husin, M.Si.
Uncontrolled Keywords: Aeromonas hydrophila GPl-04; efektivitas; kemangi; kesetaraan; kloramfenikol; minyak atsiri; Aeromonas hydrophila GPl-04; effectiveness; ocimum; equivalent; chloramphenicol; aetheric oil;
Subjects: L Education > L Education (General)
Q Science > QH Natural history
Q Science > QH Natural history > QH301 Biology
Divisions: Fakultas Keguruan dan Ilmu Pendidikan > Pendidikan Biologi
Depositing User: Riski Wismana
Date Deposited: 27 Dec 2017 00:47
Last Modified: 27 Dec 2017 00:47
URI: http://repository.ump.ac.id/id/eprint/6452

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