Background : Jeruju belongs to semi-mangrove plants which is considered as
weeds by the people in coastal area. Several researches reported that extract of
jeruju (Acanthus ilicifolius) exhibited antimicrobial activity, anticancer,
antioxidant, hepatoprotective, antiinflamation, and hemolytic. One of secondary
metabolite compound which exists in jeruju stem is lignan glucosides which is
categorized as phytoestrogens. Thus, it is interested to observe about estrogenic
activity in jeruju stem using YES-assay method.
Aims of The Research : This research was aimed to determine estrogenic activity
of water and ethyl acetate fraction of jeruju stem (Acanthus ilicifolius) and to
determine its TLC profile.
Research Methodology : Fractionation was done by using liquid-liquid
fractionation. Estrogenic assay on water and ethyl acetate fraction of jeruju stem
was done with concentration series of 50μl, 100μl, 200μl, 400μl, 800μl, 1200μl,
1600μl, 2000μl. The data was analyzed by using Graphad prism 4th version.
Identification of fraction from extract of jeruju stem was done by using Thin
Layer Chromatogram method (TLC).
Result : Water fraction exhibited the highest estrogenic activity either determined
by using YES-hERα or YES-hERβ, while ethyl acetate fraction showed the lowest
estrogenic activity either determined by YES-hERα or YES-hERβ. The value of
EC50 from water and ethyl acetate fraction determined by YES-hERα was 360,2
μg/ml and 4,369x1030 μg/ml. The value of EC50 from water and ethyl acetate
fraction determined by YES-hERβ was 1083 μg/ml, and 1,34x106 μg/ml. From
compound identification using TLC, it showed that water fraction contained
flavonoid. While within ethyl acetate fraction contained flavonoid, alkaloid,
saponin, tannin, and terpenoid.
Conclusion : Water fraction exhibited low estrogenic activity either determined
by using YES-hERα or YES-hERβ with negative relation between consentration
and β-galaktosidase unit that was produced. Whereas ethyl acetate fraction of
jeruju stem (Acanthus ilicifolius) didn’t have estrogenic activity either that
determined by using YES-hERα or YES-hERβ. Compound identification used
TLC to know the compound that was in water fraction of jeruju stem containing
flavonoid, and within ethyl acetate fraction, it contained alkaloid, flavonoid,
saponin, tannin, terpenoid.