EVI YUNITI . Identification of Antibacterial Active Compounds of Ethanolic
Combinations of Extracts Zingiberaceae Rhizome
Under Supervision of :INDRI HAPSARI, M.Si.,Apt dan BINAR ASRINING
DHIANI, M.Sc.,Apt
Background:Back to nature concept has increased the use of traditional medicine
alternative. One of thosetraditional medicines is Zingiberaceae that has been
known to have antibacterial activity.
Object :To find out chromatogram profile and to determine antibacterial activity
of ethanolic extracts combination of Zingiberaceae rhizomes againts Escherichia
coli.
Method :This study were descriptive study and experimental study. Ethanolic
extracts of Zingiberaceae rhizomes was made by maceration method with ethanol
as solvent. Thin layer chromatography was used to obtain chromatogram profile.
For antibacterial study, Escherichia coli was used. Each group was given fraction
in concentration of 2000μg/ml, 1000μg/ml, 500μg/ml, 100μg/ml, 50μg/ml,
5μg/ml.
Result :There were four compoundson thin layer chromatography. The major
compounds were flavonoid, alkaloid, tanin and terpenoid. Combination of
ethanolic extracts Zingiberaceae rhizome active as antibacterial againts
Escherichia coli.
Conclusion :The combination extracts ethanol of Zingiberaceae rhizome
contained flavonoid, alkaloid, tanin and terpenoid. All of the fraction of
combination extracts ethanol of Zingiberaceae rhizome have antibacterial activity
againts Eschericia coli.