VALIDASI METODE DAN PENETAPAN KADAR NATRIUM DIKLOFENAK DENGAN KCKT SERTA APLIKASINYA PADA PENETAPAN NATRIUM DIKLOFENAK TABLET SPESIALITE DALAM PLASMA DARAH TIKUS WISTAR JANTAN SECARA IN VITRO

AYUNINGSIH,, HERLINA (2014) VALIDASI METODE DAN PENETAPAN KADAR NATRIUM DIKLOFENAK DENGAN KCKT SERTA APLIKASINYA PADA PENETAPAN NATRIUM DIKLOFENAK TABLET SPESIALITE DALAM PLASMA DARAH TIKUS WISTAR JANTAN SECARA IN VITRO. Bachelor thesis, UNIVERSITAS MUHAMMADIYAH PURWOKERTO.

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Abstract

HERLINDA AYUNINGSIH. Method Validation and Levels Determination of Diclofenac Sodium by HPLC and Its Application to Determination Diclofenac Sodium Tablets Spesialite in Blood Plasma Male Wistar Rats in Vitro . Under guidance of SUSANTI and ASMIYENTI DJALIASRIN DJALIL The Background: The use of diclofenac sodium in addressing various problems for all kinds of pain, migraine and gout. Activities by way of inhibiting the enzyme cyclo-oxygenase that inhibited prostaglandin formation (Tjay and R ahardja, 2007). To ensure safety and minimize the side effects of a drug necessary to test the drug analysis in biological fluids (Shargel and Yu, 2005). T he Aim of Research: This research conducted to prove that the high performance liquid chromatography method is very valid to define levels of diclofenac sodium in tablets Spesialite on Wistar rat plasma in vitro . The Method of Research: Type of research method used is non-experimental , inst ruments such as HPLC UV detector (273 nm ), mobile phase of methanol : acetate buffer pH 4.2 by comparison 70:30, the stationary phase chromatography- oc tadecyl coloumliquid cylane and a flow rate of 0.7 mL / min . Standard materials used are e merck diclofenac sodium, diclofenac sodium tablets Spesialite and wist ar rat blood plasma. Validation of analytical methods include liniearitas, limit of detection, limit of quantitation, precision, accuracy, and conducted determination method diklofenakspesialite sodium in Wistar rat plasma in vitro . The Result : Linearity test results obtained with the correlation coefficient r count or value = 0.998 ( > r table 0.707 ), limit of detection = 0.2 mg / mL, the limit of quantitation = 0.6 mg / mL, accuracy or precision concentration of 4 mg / mL = 0.57 % coefficient of variation ( ≤ 2%), precision or accuracy for the addition of diclofenac sodium. 7 mg / mL average recovery of 105.4762 %, and determination of diclofenac sodium tablets 50 mg Spesialite plasma concentrations obtained with the 3 series range of levels of 51.002 mg / tablet 50.902 mg / tablet or acquired 98.51 % percent of - 102 %. Conclusion: Based on the above results it can be concluded that the high pe rformance liquid chromatography method is valid to analyze Spesialite diclofenac sodium levels in the blood plasma of Wistar rats in vitro .

Item Type: Thesis (Bachelor)
Additional Information: Pembimbing: Susanti, M.Phil, Apt dan Dr. Asmiyenti D.D, S.Si, M.Si
Uncontrolled Keywords: natrium diklofenak, validation method, high performance liquid chromatography, plasma, in vitro.
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Fakultas Farmasi > Farmasi
Depositing User: eko wahyudi
Date Deposited: 20 Jul 2017 02:15
Last Modified: 20 Jul 2017 02:15
URI: http://repository.ump.ac.id/id/eprint/2835

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