PENENTUAN AKTIVITAS ESTROGENIK EKSTRAK ETANOL DAUN DAN BUNGA JERUJU (Acanthus ilicifolius) MENGGUNAKAN YES-ASSAY

SARI, DIAN KARTIKA (2014) PENENTUAN AKTIVITAS ESTROGENIK EKSTRAK ETANOL DAUN DAN BUNGA JERUJU (Acanthus ilicifolius) MENGGUNAKAN YES-ASSAY. Bachelor thesis, UNIVERSITAS MUHAMMADIYAH PURWOKERTO.

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Abstract

Background: Estrogen is a steroid hormone that is produced mostly in women and produced in small amounts in men. Some diseases associated with lack of estrogen in the body can occur in women, especially in women who experience post menopause. Objective: This study aimed to determine the estrogenic activity of the leaves and flowers ofJeruju (Acanthus ilicifolius) and EC50 of estrogenic activity were determined using YES-assay. Method:This study performed by in vitro using YES- assay. With series of concentration of ethanol extract from leaf and flower of jeruju (Acanthus ilicifolius) 50,100,200,400,800,1000,1200,1600,2000 μg/ml in DMSO. Results: The ethanol extract of leaves of Jeruju (Acanthus ilicifolius) on YES - hERα showed that the treatment by extract was proportional to amount of units β- galactosidase. At a concentration of 50 ug / ml showed the lowest activity. And at highest concentration 2000 ug / ml showed the highest β galactosidase enzyme activity. EC50 leaf of ethanol extract from jeruju samples determined byYEShERα was 28.960 μg / ml. While the results from ethanol extract of the leaves YES - hERβ showed the lowest β-galactosidase activity in the smallest concentration (50 ug / ml), and the largest activity at a concentration of 2000 ug / ml. EC50 for ethanol extract of leaf samples determined by YES - hERβ was 29.292 ug / ml. In addition, the ethanol extract of flowers determined byYEShERα exhibited the yelow colours from oNP. However, EC50 ethanol extract of flowers could not be calculated based on the parameters of the four models of log dose - response. While on YES - HERβ treated by ethanol extract of flowers exhibited β-galactosidase enzyme activity at concentration of 100 ug / ml and the highest concentration of 2000ug / ml. So the EC50 obtained for 1,809 x 108 ug / ml. Conclusion: The ethanol extract of leaves and flowers weeds exhibited very weak estrogenic activity as determined by YES-hERα and YES-hERβ.

Item Type: Thesis (Bachelor)
Additional Information: Pembimbing: Binar Asrining Dhiani, M.Sc., Apt. dan Susanti, M.Phil., Apt.
Uncontrolled Keywords: Yes- assay; Estrogen receptor α (ER α); Estrogen receptor β (ER β); phytoestrogens; Acanthus ilicifolius
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Fakultas Farmasi > Farmasi
Depositing User: Danarto Kh
Date Deposited: 13 Jul 2017 00:31
Last Modified: 13 Jul 2017 00:31
URI: http://repository.ump.ac.id/id/eprint/2625

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