PENELUSURAN PENYEBAB RESISTENSI ANTIBIOTIKA DARI BAKTERI MULTIRESISTEN ANTIBIOTIK (ISOLAT MG 46 DAN WK 45)

SIWI, PRITARANI AULIA (2015) PENELUSURAN PENYEBAB RESISTENSI ANTIBIOTIKA DARI BAKTERI MULTIRESISTEN ANTIBIOTIK (ISOLAT MG 46 DAN WK 45). Bachelor thesis, UNIVERSITAS MUHAMMADIYAH PURWOKERTO.

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Abstract

An increase in infectious diseases due increase in bacterial resistance to antibiotics. Resistance can occur extrachromosomal or chromosomal. This study aims to determine the cause of antibiotic resistance in bacteria multiresistant MG 46 and WK 45. MG 46 resistant to chloramphenicol and gentamicin and WK 45 resistant to amoxicillin and chloramphenicol. At isolates MG 46 and WK 45 did not found plasmid, thus we analyze the chromosomal DNA. Chromosome analysis was done by PCR using universal primer 16S rRNA and after that the results of PCR restricted use the enzyme PstI to be cut sequences CTGCAG. Restriction fragment of differences in results between the MG 46 and Bacillus cereus, that there are two fragments of the MG 46 (∞ 700 bp and ∞ 900 bp) and the absence of fragme nts on Bacillus cereus. The easiest possibility that replacement bases adenine become cytosine at nucleotide number 7004 so that the sequence CTGAAG become CTGCAG that can be recognized by PstI. And the results of Streptococcus pneumoniae restriction can not be used to compare with the results of restriction WK 45 because the result Streptococcus pneumoniae restriction experienced star activity when compared with the NCBI database. Could be concluded possibility of mutations in the 16S rRNA MG 46 that causes can be recognized by the enzyme PstI.

Item Type: Thesis (Bachelor)
Additional Information: Pembimbing: Binar Asrining Dhiani, M.Sc., Apt. dan Susanti, M.Phil,. Apt.
Uncontrolled Keywords: cause of resistance, MG 46, WK 45, chromosome analysis, 16S rRNA
Subjects: R Medicine > RM Therapeutics. Pharmacology
Divisions: Fakultas Farmasi > Farmasi
Depositing User: Danarto Kh
Date Deposited: 24 May 2017 00:49
Last Modified: 24 May 2017 00:49
URI: http://repository.ump.ac.id/id/eprint/1974

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