ISOLASI DAN IDENTIFIKASI BAKTERI YANG TOLERAN TERHADAP FUNGISIDA TILLO DAN INSEKTISIDA REGENT PADA TANAH SAWAH PERTANIAN PADI DI DESA CISALAK-CIMANGGU

DARMUTI, . (2017) ISOLASI DAN IDENTIFIKASI BAKTERI YANG TOLERAN TERHADAP FUNGISIDA TILLO DAN INSEKTISIDA REGENT PADA TANAH SAWAH PERTANIAN PADI DI DESA CISALAK-CIMANGGU. Bachelor thesis, UNIVERSITAS MUHAMMADIYAH PURWOKERTO.

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Abstract

This research aimed to isolate and identify tolerant bacterium towards the fungicide Tillo and insecticide regent and to find out their ability to degrade the pesticide. The isolation was done by dilution up to 10-7 continued to pure plate on medium nutrient in order to get 1% fungicide Tillo and 1% insecticide Regent. The obtained pure isolates were identified based on the observation of colonial macroscopic, microscopic cells, and bio chemical test. The isolates bacterium was identified by using the book of Bergey‟s Manual Determinative of Bacteriology (Holt et al., 1994), and supported articles of Lestari et al., (2016), Patasik dan Lantang (2009), Felitra et al., (2004), Setiyaningrum (2011), Wiluyandari (2013), Bintari (2015), Azizah (2016), Marista (2013), Maulida (2010) Fatuni (2014) and Yulvizar (2013). The identification results show there are 12 bacterium genuses, Morganella, Klebsiella, Aeromicrobium, Xenorhabdus, Plesiomonas, Lactobacillus, Citrobacter, Erwinia, Micrococcus, Ampibacilus, Hafnia, and Vibrio. The findings of this research show that there are six tolerant bacterium genuses toward the fungicide tillo which are Morganella, Klebsiella, Aeromicrobium, Xenorhabdus, Plesiomonas and Lactobacillus, and there are seven tolerant bacterium genuses toward the insecticide regent which are Klebsiella, Citrobacter, Erwinia, Micrococcus, Ampibacilus, Hafnia, and Vibrio. Bacterium challenge test was done to fungicide Tillo and insecticide Regent with 2.5%, 5%, and 10% concentrate for each. The formed data of isolates and clear zone were then qualitatively and descriptively analyzed. Two bacterium genuses are able to degrade insecticide regent which are Klebsiella in 2.5% concentrate and Erwinia in 5% concentrate. All the tolerant bacterium toward fungicide Tillo are not able to degrade (forming clear zone) from fungicide Tillo.

Item Type: Thesis (Bachelor)
Additional Information: Pembimbing: Drs. Heri Maryanto, M.Si.
Uncontrolled Keywords: Bacterium, Tillo, Regent, Paddy Farmland, Tolerance
Subjects: Q Science > QH Natural history > QH301 Biology
Depositing User: Danarto Kh
Date Deposited: 20 Apr 2017 02:20
Last Modified: 20 Apr 2017 02:20
URI: http://repository.ump.ac.id/id/eprint/1688

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